Optical Activity of Sugar
Materials: ★★★ Requires materials not commonly found in school laboratories
Difficulty: ★★☆ Can be done by science teachers
Safety: ★☆☆ Minimal safety procedures required
Categories: Organic Chemistry, Light
Alternative titles: Rotation of Polarized Light by Sugar Solution
Summary
This demonstration shows that sugar solutions are optically active and can rotate plane-polarized light, while water cannot. Using polarizing filters and an overhead projector, the optical activity of sugar is made visible.
Procedure
- Cut two square holes in each cardboard sheet, spaced evenly apart.
- Tape polaroid films over the holes, ensuring one sheet has the polarizing direction at 90° to the other.
- Dissolve as much sugar as possible in 200 mL water in one beaker, heating to make a concentrated syrup. Leave the other beaker with plain water.
- Place one polaroid sheet on the overhead projector and set the beakers on top, each over one of the polarized light squares.
- Observe both solutions as light passes through.
- Place the second polaroid sheet above the beakers aligned with the first sheet at 90°.
- Observe that light only passes through the sugar solution, showing optical rotation.
Links
Optical Rotation Demonstration Polaroid and Glucose - Farnborough Chemistry:
📄 Optical activity of sugar (Demonstration 17, page 36) - Dr Magdalena Wajrak and Mr Tim Harrison: https://www.ecu.edu.au/__data/assets/pdf_file/0003/910443/Chemical_Demonstration_Booklet_Interactive_Final_14.2.21.pdf
Variations
- Use glucose or fructose instead of sucrose and compare the degree of rotation.
- Try different concentrations of sugar to see how the brightness changes.
- Replace the overhead projector with a laser pointer and polarizing lenses for a smaller-scale version.
Safety Precautions
- Handle the hot plate carefully to avoid burns.
- Prevent sugar solution from boiling over onto the hot plate.
- Allow hot sugar solution to cool before disposal.
- Wear safety glasses to protect against splashes.
Questions to Consider
- Why does light pass through the sugar solution but not through plain water? (Because sugar is optically active and rotates polarized light, while water is not optically active.)
- What property of sugar molecules makes them optically active? (Their chirality – sugar molecules lack symmetry and exist in enantiomeric forms.)
- How would the demonstration change if a racemic mixture of sugar is used? (There would be no net rotation of polarized light, so no light would pass through.)
- What happens if you increase the concentration of the sugar solution? (The rotation of light increases, making the transmitted light brighter or more visible.)